LOCUS       40924_13765        4861 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCtrlki-kanMX6 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Takeda J, Taoka M, Isobe T.
  TITLE     Cloning vector pCtrlki-kanMX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Takeda J, Taoka M, Isobe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2011) Contact:Jun Takeda Tokyo Metropolitan 
            University, Graduate School of Science and Engineering; 1-1 
            Minami-osawa, Hachioji, Tokyo 192-0397, Japan
REFERENCE   3  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2011) Contact:Jun Takeda Tokyo Metropolitan University, 
            Graduate School of Science and Engineering; 1-1 Minami-osawa, 
            Hachioji, Tokyo 192-0397, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    231..266
                     /label=Multiple cloning site 1
                     /note="Multiple cloning site 1"
     polyA_signal    391..510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            556..1912
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     misc_feature    1947..1976
                     /label=Multiple cloning site 2
                     /note="Multiple cloning site 2"
     primer_bind     complement(1990..2006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2219..2674
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2956..3060
                     /label=AmpR promoter
     CDS             3061..3918
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4092..4680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcggta
      241 ccgtcgaccc cggggagctc catatgtgag cggccgcgac tctagatcat aatcagccat
      301 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      361 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      481 tgtggtttgt ccaaactcat caatgtatct ggatctgttt agcttgcctc gtccccgccg
      541 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      601 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
      661 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
      721 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
      781 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
      841 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
      901 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt
      961 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     1021 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1081 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     1141 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     1201 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1261 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1321 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1381 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1441 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1501 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1561 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1621 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1681 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt
     1741 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     1801 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     1861 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     1921 tcgatactaa cgccgccatc cagtttgata tcggatctac tagtctcgag gcatgcaagc
     1981 tagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2041 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2101 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2161 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     2221 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2281 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2341 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2401 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     2461 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2521 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     2581 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2641 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     2701 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2761 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2821 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2881 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4801 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4861 g
//
